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pneumoniae were analyzed, it was observed that the union between the enzyme and the cell wall was always accomplished through the choline residues present in the teichoic acids of the cell wall (Jado et al 2003) The entity responsible for the recognition and union is a six-repeat motif located at the cell wall-anchoring domain (Hermoso et al 2003) It was also observed that a minimum of four repeats of choline-binding motif was necessary for ef cient binding to the cell wall (Lopez and Garcia 2004) It was proposed that the amino alcohol would serve as an element of selective pressure to preserve the substrate-recognition domain Recently we have elaborated a protein sequence-based phylogenetic tree with known lysin sequences of bacteriophages able to recognize the amino alcohol motif The tree was elaborated employing the Neighbor-Joining algorithm with Kimura corrections (Fig 41).

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workforce two decades ago; today they are 185 percent of workers 22 million people according to the Bureau of Labor Statistics Why not outsource Some people hesitate for the following reasons: They think they re too small to need or afford help They don t have enough work to keep an assistant busy They don t make enough money to be able to afford to pay someone else to do some of their work But as you become busier, you realize that the amount of work you can do, and therefore the amount of income you can generate, is limited by your own energy and the number of hours you can work in a day One way around this is to spend more of your time on billable work, especially work that earns a high hourly rate.

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When the tree was analyzed, it was observed that lysin sequences were present in phage and bacteria genomes The presence of lysins in bacteria occurs because these enzymes are necessary during normal cell wall extension, the separation of daughter cells, cell motility, and so on It has been suggested that endogenous lytic cell wall enzymes would be related to the irreversible effect of -lactam antibiotics (Tomasz 1979) The high similarity found between cells and phage lysins can be taken as a proof of the selective pressure to preserve substrate-recognition domains It is noteworthy that the lysins able to act against Streptococcus species are not directly related to any other lysin This property must be due to the aforementioned presence of the six-repeat motif located at the C-terminus binding domain The high similarity between the genes of phages infecting Streptococcus pyogenes and S.

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pneumoniae suggests a frequent genetic interchange between both species and the recent divergence from a common ancestor phage (Obregon et al 2003) Interesting proof of the above selective pressure in lysin-binding domains is the enzyme Pal This is an amidase with lytic activity against pneumococci The protein exhibits the typical dual organization of lysins: a catalytic domain with amidase activity and a binding domain with a six-repeat motif The catalytic domain has no homology with the rest of the Streptococcus amidases However, the binding domain is well conserved (Varea et al 2004) It is possible that a natural recombination process, a catalytic domain from an unknown precursor and the binding domain acquired from S pneumoniae, is the origin of this enzyme Analysis of the phylogenetic relationships between phage and bacterial genomes suggests that the former may have evolved by recombi-.

To do this, you have to free up some time by not doing work that is not billable or is billed at a low rate This is where hiring outside help comes in You make money by thinking and producing Everything else learning how to use a particular computer program, scanning source materials, going to the library, buying supplies is a waste of time that could be spent on productive work Some or all of these nonessential activities can be outsourced to others What should you outsource Businesspeople outsource all different kinds of tasks including the following: Research Filing Typing and word processing Mailing list and database management Telephone selling and telemarketing Customer service (help desk) Proofreading Sales and marketing (through agents) Bookkeeping and accounting Computer programming You can outsource all or some of these tasks at your discretion.

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