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from the lumen and then allows potassium to enter the interstitium across the basolateral membrane via potassium channels Regulation of potassium excretion involves multiple controls over the secretory processes in the distal nephron, something like a passenger van where everyone in it has an accelerator and a brake pedal As is the case with regulation of sodium excretion, we cannot predict just how these controls operate in every situation Fortunately, with potassium as well as sodium, the healthy kidneys do a remarkable job of doing the right thing, ie, increasing potassium excretion in response to high dietary loads and reducing excretion in the face of restricted diets Even with experimental manipulations, it is hard to get healthy kidneys to do otherwise (but as described later, certain pathologies interfere with normal potassium regulation) Much of the regulation involves controlling the activity of potassium channels The kidneys and other body organs express numerous potassium channel species; for simplicity, we do not usually differentiate between types (Otherwise, we would have a whole book devoted entirely to potassium channels!) However in principal cells of the distal nephron two types of channels stand out as being those that secrete potassium in a regulated manner: ROMK (standing for renal outer medulla, because that is where they were first indentified) and BK (as each channel has a big capacity to secrete potassium) Owing to different combinations of channel subunits and alternative splicing of transcripts, several isoforms of each type of channel exist Although ROMK and BK channels both conduct potassium, they play different roles and are regulated by quite different mechanisms5 At very low dietary loads of potassium, there is virtually no secretion by either kind of channel ROMK channels are sequestered in intracellular vesicles and BK channels are closed At normal potassium loads, ROMK channels are moved to the luminal membrane and secrete potassium BK channels are still closed, held in reserve and ready to respond to appropriate signals when needed At high excretion rates, both types of channel are present in the luminal membrane and avidly secreting potassium (Figure 8 3) Figure 8 4 shows factors known to influence the secretion, and thus the ultimate excretion of potassium The following text provides a brief description of how specific factors affect potassium excretion (1) Plasma potassium The role of plasma potassium is the most understandable influence First, the filtered load is directly proportional to plasma concentration Second, the environment of the principal cells, ie, the cortical interstitium, has a potassium concentration that is nearly the same as in plasma The Na-K-ATPase that takes up potassium is highly sensitive to the potassium concentration in this space, and varies its pump rate up and down when potassium levels in the plasma vary up and down Thus, plasma potassium concentration does exert an influence on potas5 BK channels require either an elevation in intracellular calcium or a large membrane depolarization in order to open them ROMK channels are regulated by a multitude of intracellular signaling cascades Some affect expression of the channels, some move channels back and forth between the luminal membrane and storage vesicles, while others alter the probability that the channel is in an open configuration Although progress is being made in sorting out the details of these intracellular cascades, it is still a challenge to relate them to extrarenal signals that turn them on and off.

the British government $35 billion for access to Britain s third-generation radio spectrum located in the 2 GHz band Germany followed suit with auctions garnering over $50 billion In both cases, the mobile operators paid these sums to license the air; all of the costs of network build-out are completely additive to these costs! These bidding wars anticipate the wide-scale adoption of users who will gravitate towards mobile computing for every possible type of application for work and pleasure Internationally, the 3G standard is codified as IMT-2000 This standard will support wireless multimedia and large file transfers at theoretical speeds up to 2 Mbps per second When deployed, IMT-2000 will harmonize two competing standards for 3G, the North American standard CDMA-2000 (an outgrowth of the Qualcomm-supported IS-95, used in current CDMA voice networks), and the European/Japanese wideband CDMA standard Ultimately, consumers of wireless phone and data services should be able to talk" and communicate data seamlessly across all 3G networks if harmonization is achieved Consider for a moment what 2 Mbps will mean Although slower than most LAN connections, 2 Mbps will be able to deliver data at a rate that will make streaming video smooth That means that teenagers who want to watch their favorite music videos will be able to request them from a web site and have them sent to them at a party or walking down the street Clearly this is a far cry from where the state of the art is today, however, it is obvious why there is so much excitement surrounding the rollout of 3G networks 25 G is the Reality Today In the meantime, the first generation of 25G networks is beginning its commercial deployment These networks, while not achieving the blistering speeds promised by 3G, will offer enough bandwidth for mobile users to support very rich business applications The interim 25G standards include General Packet Radio Standard (GPRS), a 115 Kbps packet radio standard designed for GSM networks Select deployments of GRPS are beginning in Europe and North America Among CDMA carriers, the 25G standard known as CDMA 1XRTT will be deployed 1XRTT will provide users with nominal data rates of 144 Kbps, enough to support graphics, audio, text, large files, and more Another standard, Enhanced Data For Global Evolution (EDGE), considered an evolution of Time Division Multiple Access (TDMA), has promised speeds to 384 Kbps, but appears to be eclipsed by GPRS and wideband CDMA The EDGE standard was originally supported by AT&T Wireless, which now enjoys significant investment from NTT DOCOMO in Japan In Japan, the most.

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Each man must contribute to the spirit and this spirit is really the cohesive force that binds 11 hardened and talented men into a cohesive force Just as you must contribute to the spirit of your company, to the spirit of your associates

BK ROMK (sequestered) (closed)

Bart Starr offers an insider s perspective: Lombardi explained that some of the players on the team were going to be famous, some obscure, but everyone was equally important For us to succeed, we had to place our personal goals behind those of the team We had to pick each other up and push each other to higher levels

aggressive nation to adopt wireless data, wideband CDMA will be deployed over the next several years In Europe, 15 countries in the European Union must begin IMT-2000 deployments, by law, no later than January 2002, and 3G licensing is already taking place in New Zealand, Germany, South Africa, and the Netherlands, according to officials at the Global Mobile Suppliers Association Among most GSM operators and there are over 400 in 42 countries (GSM now commands 450 million subscribers worldwide) most are already planning on packet-based GPRS deployments for Internet support and high-speed wireless As many as 80 to 100 licenses for wideband CDMA (IMT-2000) will be purchased worldwide At the beginning of the migration, most GSM operators will go with GPRS, some will choose EDGE at 384 Kbps for data, and some will stop at EDGE and not move on to 3G But others will wait for IMT-2000 because they're in the bigger markets and need the network capacity

ROMK (open)

LOMBARDI RULE #10

BK (closed)

Build team spirit This means common goals, complementary skills and abilities, and mutual accountability

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