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As your site grows in complexity, keeping track of the various links is an increasingly difficult task. Dreamweaver has several enhanced features to help you manage links, and the Update Links When Moving Files option is one of them. Dreamweaver can check each link on a page when a file is moved whether it is the Web page you re working on or one of the support files, such as an image, that goes on the page. The Update Links option determines how Dreamweaver reacts when it notes an altered link. By default, the Update Links When Moving Files option is set to Prompt, which causes Dreamweaver to alert you to any link changes and requires you to verify the code alterations by selecting the Update button. To leave the files as they are, choose the Don t Update button. You can elect to have Dreamweaver automatically keep your pages up to date by selecting the Always option from the Update Links drop-down list. Finally, you can select the Never option, and Dreamweaver ignores the link changes necessary when you move, rename, or delete a file. As a general rule, I keep my Update Links option set to Always. It is a very rare circumstance when I intentionally maintain a bad link on my Web page. Likewise, I recommend using the Never option with extreme caution.

of the electrical power delivered to the thruster that must be accounted for in the total discharge cost of the subsystem. The plasma is typically a difficult load to match well, and reflection of 10% to 30% of the microwave energy back into the recirculator (which absorbs the reflected power from the source in the case of mismatch or faults) is typical. The microwave source and recirculator usually represent a significant mass and volume addition to the ion thruster system. An examination of Table 4-1 shows that, in order to avoid cutoff and produce ion current densities to the grids of 1 to 2 mA/cm2, microwave sources in the 4- to 6-GHz range are required. At this time, space TWTs in this frequency range are limited in power capability to the order of a few hundred watts. For a given discharge loss, this limits the total ion current that can be produced by a microwave thruster. While microwave thrusters hold the promise of eliminating the need for thermionic cathodes used in DCdischarge thrusters and of doing away with the requirement for dielectric discharge chambers in rf thrusters, producing high-efficiency, high-thrust ion propulsion systems based on this technology can be challenging. This is certainly an area for future research.

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If you want to reverse playback, select the Reverse check box. Again, press Spacebar to preview the effect. Click Done when you re satisfied with the result. iMovie closes the Inspector dialog box. You can type a custom value in the percentage box (for example, 173%) to make a clip play at exactly the speed (or for exactly the time) you need. Generally, it s better

(16-11)

CH,Cl" -ISOC (I h), RT (I h)

C =Iv(i$ - gv ,

Looking at Figure 13-13, you can see that there are several tabs on the left side of the Format Plot Area dialog box that are available for formatting the plot area. This dialog box in Figure 13-13 is available for several other components and areas of your PivotChart. All you need to do is right-click one of them and select the format item from the pop-up menu.

5.3.5. Maintenance of Gonad-Derived Cell Cultures It is our practice to include antibiotics in all our gonad-derived primary cultures, at least in part relating to the nonsterile source of the starting material. We have not found it necessary to use antifungal agents. However, the ever-present risk of mycoplasma infection necessitates the initiation and maintenance of early cultures in a dedicated incubator. Once a culture is established, routine testing for mycoplasma should be carried out; for instance, concurrent with characterization by karyogram (see Protocol 5.9). We have used a variety of the commercially available methods and have developed no particular preference. Cultures are left undisturbed for the rst 2 days to allow gonadal cells, including the germ cell lineage, to attach to feeders. As with ESC culture, no speci c methodology is mandatory. Our rationale for minimal intervention is to allow cells to adjust to a radically altered environment without further insult. After 2 days, the cultures may contain a lot of cell debris that can be removed by complete medium exchange. Alternatively, where cultures appear to contain fewer dead cells, half the medium can be exchanged to preserve more conditioning from the feeder cells. Thereafter, cultures should be brie y monitored daily, concurrent with medium exchange, to minimize time out of the incubator. As well as observing proliferation of the culture, this opportunity allows evaluation of the feeder cell integrity. These criteria determine the timing of the rst passage, carried out during the second week, usually between days 9 and 11 of culture. Analysis of culture samples is carried out thereafter, as appropriate (see Section 5.4).

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