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251 Equipment Used The modes of chromatography described in Section 24 may be performed in a chromatography system operating at atmospheric pressure, using stationary phase particles with relatively large (eg 50 150 M) diameters Such a system is called open column chromatography or low performance liquid chromatography This gives relatively low resolution and is slow compared to the more high-resolution systems described in the following sections (Sections 26 29) However, there are also a number of compelling advantages to the use of open column chromatography Stationary phases and other equipment used are cheap making this approach especially convenient for large-scale preparative chromatography This lends itself to steps used early in protein puri cation procedures (Section 2101), where a priority is the decrease of total protein in the extract to facilitate subsequent application to high-resolution systems.

Figure 9-15: Selecting objects with a crossing window. The crossing window selects any objects that lie within or partly within the window.

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Columns and tubing used at low pressure may be constructed out of cheap and readily available materials Generally, plastic reservoirs and tubing are used for mobile phase Stationary phases are retained in glass or plastic columns either by a high porosity glass sinter or else by placing plastic wool at the bottom of the column A degree of standardization of ow rate is possible with a peristaltic pump although ow rates achieved will be limited by the hydrodynamic properties of the stationary phase Since this is generally composed of polysaccharide-based gels that are quite soft, they are limited in the hydrostatic pressures which they can accommodate without compression Most systems ow adequately under gravity although the ow rate may vary somewhat during chromatography as mobile phase level in the reservoir drops (Figure 219) When using gravity ow, a safety loop is usually included in the system design.

Abbott, C.R., Rossi, M., Wren, A.M. et al. (2001) Evidence of an orexigenic role for cocaine- and amphetamine-regulated transcript after administration into discrete hypothalamic nuclei. Endocrinology, 142, 3457 63. Adan, R.A., Tiesjema, B., Hillebrand, J.J. et al. (2006) The MC4 receptor and control of appetite. British Journal of Pharmacology, 149 (7), 815 27 Epub 2006 Oct 16. Ahima, R.S. and Flier, J.S. (2000) Leptin. Annual Review of Physiology, 62, 413 37. Ahima, R.S., Dushay, J., Flier, S.N. et al. (1997) Leptin accelerates the onset of puberty in normal female mice. Journal of Clinical Investigation, 99, 391 5. Aja, S., Sahandy, S., Ladenheim, E.E. et al. (2001) Intracerebroventricular CART peptide reduces food intake and alters motor behavior at a hindbrain site. American Journal of Physiology. Regulatory Integrative and Comparative Physiology, 281, R1862 7. Akira, S. (2000) Toll-like receptors: lessons from knockout mice. Biochemical Society Transactions, 28(Part 5), 551 6. An, J., Muoio, D.M., Shiota, M. et al. (2004) Hepatic expression of malonyl-CoA decarboxylase reverses muscle, liver and whole-animal insulin resistance. Nature Medicine, 10, 268 74. Anand, B.K. and Brobeck, J.R. (1951) Localization of a feeding center in the hypothalamus of the rat.

3. Uncheck Specify On-screen in the Insertion point, Scale, and Rotation sections if you want to specify the insertion point, scale, and rotation angle in the dialog box. Then provide the requested information in the dialog box.

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